The purification effect of different models resin and their activity in vitro of the total saponins in fruit of Rosa davuvrica Pall. were studied. Eight types of macroporous resins, such as LSA-21, D-101 and AB-8, were used to purify the total saponins from Fruit of Rosa davuvrica Pall.. Adsorption rate and desorption rate were determinated by ultraviolet-visible spectrophotometry method. The results showed that the LSA-21 type of macroporous resin had the best purification effect on the total saponins in fruit of Rosa davuvrica Pall..The best conditions were as follows: The concentration of adsorption liquid was 0.1927 g/mL. The volume of adsorption liquid was 25 mL. The pH of adsorption liquid was 6.5. The volume fraction of ethanol was 90 %. The volume of desorption liquid was 40 mL and the desorption solution pH was 8.5. With the above purification conditions, the calculated adsorption and desorption rate of the total saponins in fruit of Rosa davuvrica Pall. could reach 77.51 % and 65.28 %, respectively. The content of the total saponins in dry extract was increased from 9.49 % to 33.65 %. The test results showed that scavenging ability to DPPH·, ·OH, superoxide anion and ABTS free radicals, vitro antioxidant activity, and the inhibition of the activity of alpha glucosidase can be benefited from the total saponins in fruit of Rosa davuvrica Pall.. The results provide a reference to further study on the total saponins in fruit of Rosa davuvrica Pall..
刘金璐, 雷永平, 王晓林, 钟方丽. 刺玫果总皂苷的纯化工艺及体外活性研究 [J]. 兴发pt登录学报, 2018, 35(9): 16-23. LIU Jin-lu, LEI Yong-ping, WANG xiao-lin, ZHONG fang-li. Study on the Purification Method of Total Saponins fromFruit of Rosa Davuvrica Pall. and its Purification Method . Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 16-23.