A suitable extraction method was selected to extract the antibacterial substance from bitter melon and the bioactivity was analyzed in this text. The effective method is extracting with 70 % ethanol, breaking with ultrasonic, extracting with n-butyl alcohol, and then using different concentrations of ethanol for elution from polyamide column. The results of the experiments indicated that the crude extract, the n-butyl alcohol extraction phase and the elution showed obviously antibacterial activity to Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The extracts in this text of bitter melon obviously cleared the H2O2. Qualitative analysis showed that the extracts contained saponins, flavonoids, polyphenols and organic acids.
葛雅琨, 张亚奇, 于明, 荣家闵, 张元新. 苦瓜中抑菌物质的提取及其生物活性初步分析 [J]. 兴发pt登录学报, 2018, 35(9): 88-92. GE Ya-kun, ZHANG ya-qi, YU Ming, RONG Jia-min, ZHANG Yuan-xin. Extraction and Biological Activity Preliminary Analysis of Antimicrobial Substance from Bitter Melon . Journal of Jilin Institute of Chemical Technology, 2018, 35(9): 88-92.